Wolfgang Marwan, Magdeburg, GER
from 13:00 to 15:00
|Where||C 311, Albert-Einstein-Str.21|
|Contact Name||Wolfgang Marwan|
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Reconstructing the Network Controling Commitment to Cell Differentiation in the Eukaryote Physarum polycephalum
Lehrstuhl für Regulationsbiologie, Otto-von-Guericke-Universität and Max-Planck-Institut für Dynamik komplexer technischer Systeme, Sandtorstraße 1, 39106 Magdeburg, Germany
Somatic complementation by fusion of two mutant cells and mixing of their cytoplasms occurs when the genetic defect of one fusion partner is cured by the functional gene product provided by the other. We have found that complementation of mutational defects in the network mediating stimulus-induced commitment and sporulation of Physarum polycephalum may reflect time-dependent changes in the signaling state of its molecular building blocks. Network perturbation by fusion of mutant plasmodial cells in different states of activation, and the time-resolved analysis of somatic complementation effects can be used to systematically probe network structure and dynamics. Time-resolved somatic complementation quantitatively detects regulatory interactions between the functional modules of a network, independent of their biochemical composition or subcellular localization, and without being limited to direct physical interactions. The regulatory network controling commitment and sporulation is reconstructed from experimental results using a hierarchical stochastic Petri Net-based modeling and simulation framework. The Petri Net consistently represents network structure and dynamics and quantitatively reproduces genetic, biochemical, physiological and time-resolved somatic complementation data [1-3]. In cooperation with A. Wagler and R. Weismantel (Magdeburg), we have most recently developed a proof-based exact mathematical procedure for the automatic reconstruction of networks from experimental time series data.
1. Marwan, W. 2003. Detecting functional interactions in a gene and signaling network by time-resolved somatic complementation analysis BioEssays. 25:950-960.
2. Marwan, W. 2003. Theory of time-resolved somatic complementation and its use for the analysis of the sporulation control network of Physarum polycephalum Genetics. 164:105-115.
3. Marwan, W., A. Sujatha, and C. Starostzik 2005. Reconstructing the regulatory network controling commitment and sporulation in Physarum polycephalum based on hierarchical Petri net modeling and simulation J. Theor. Biol. 236:349-365.