Simone Frey, Gothenburg, Sweden
Hosts: Mattias Goksör
Institution: University of Gothenburg, Gothenburg, Sweden
Time: 09/02/2009 - 11/30/2009
In my PhD research I work on mathematical modeling of the Snf1 pathway. One of the key players of this pathway is the transcription repressor Mig1. However, measuring its intensity using Western blot technique is not yet possible due to the nonexistence of the antibody for phosphorylated Mig1. Upon glucose concentration, the protein Mig1 shows shuttling dynamics between the nucleus and the cytosol. These dynamics can be followed using fluorescence microscopy. The data acquired are intensity data on Mig1-GFP. This work was done by the group of Mattias Goksör.
During my internship I was introduced to the data generation of fluorescence microscopy. For the experimental setup the main components are: the optical imaging of the cellular response (Mig1-GFP shuttling) by a fluorescence microscope, the control of the cells and the environment which was realized by optical manipulation, and microfluidic devices. Based on this understanding, the intensity data had to be processed in order to become usable for mathematical modeling which was my main task during my stay.
As an outcome of my stay at the group of Mattias Goksör, I have new experimental data for my Snf1 pathway model. Therefore, my work has benefited a lot from my visit. I met with Mattias Goksör regularly for discussions. I also enjoyed the kind atmosphere within the group.
- Frey S, Sott K, Smedh M, Millat T, Dahl P, Wolkenhauer O & Goksör M (2010): A mathematical analysis of nuclear intensity dynamics for Mig1-GFP under consideration of bleaching effects and background noise in Saccharomyces cerevisiae. Molecular BioSystems.