Benjamin M. Bader
|Title of PhD thesis:|| ||Spatio-temporal control of Wnt/β-catenin signaling during fate commitment of human neural progenitor cells|
|Reviewers:|| ||Dieter G. Weiss, University of Rostock, Rostock, Germany|
| ||Ernest Arenas, Karolinska Institute, Stockholm, Sweden|
| || |
The Wnt/β-catenin pathway is known to be involved in neural stem cell self-renewal and neuronal differentiation. Further understanding of this neurogenesis is a prerequisite of cell replacement therapies in neurodegenerative diseases such as Parkinson’s disease. In this context, the role of Wnt/β-catenin signaling was studied in this thesis. The results presented herein indicate that Wnt/β-catenin signaling: (1) is directly connected with the degree of neuronal differentiation in ReNcell VM. (2) -activity includes a mechanism controlling the kinetics of nuclear β-catenin import. (3) -activation affects neurons stronger than non-neurons during the time of fate commitment. (4) mediates cell cycle exit or re-entry and, hence, influences the number of proliferating cells that are committed to the neuronal lineage already (5) increases during dopaminergic differentiation which was shown for the first time both in vitro and in vivo. This study substantiates the presently limited knowledge about neurogenesis in human cell models and closes the gap between the mechanistic knowledge based on rodent models and the use of human neural stem cells for cell replacement strategies in neurodegenerative diseases.
- Benjamin M Bader, Bärbel Redlich and Dieter G Weiss (2008): Quantitative 3D analysis and evaluation of confocal images for the study of translocation of Wnt-signalling molecules in human neural progenitor cells . - Poster, Marburg/Lahn, Germany, "German Society for Cell Biology - Annual Meeting 2008", published in European Journal of Cell Biology, Volume 87S1, Suppl. 58, p 18, ISSN 0171-9335..
- Benjamin M. Bader and Dieter G. Weiss (2008): Signal protein translocation in differentiating human neural progenitor cells: 3D image quantification for a systems biology approach. - In: Cytometry Part B: Clinical Cytometry, vol. 74B, pp. 383, DGfZ, Wiley InterScience. DGfZ-ESCCA joint abstracts from The 18th Annual Meeting of The German Society of Cytometry (DGfZ) and The 8th Euroconference of Clinical Cell Analysis by The European Society for Clinical Cell Analysis (ESCCA).
- Benjamin M Bader, Ernest Arenas and Dieter G. Weiss (2009): Quantitative image cytometry: nuclear beta-catenin shuttling during differentiation of human and mouse midbrain progenitor cells. - In: Abstract book: "19th Annual Conference of the German Society for Cytometry", pp. 18, Leipzig, DGfZ.